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Anti -Apg10 (Autophagy-related Protein 10, Autophagy 10-like, APG10-like, Atg10)
Anti -Apg10 (Autophagy-related Protein 10, Autophagy 10-like, APG10-like, Atg10)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B |
|
| Catalog # | A2298-75D1 | | ATG10 is an E2-like enzyme involved in two ubiquitin-like modifications essential for autophagosome formation | ATG12-ATG5 conjugation and the modification of a soluble form of MAP-LC3, a homolog of yeast Apg8, to a membrane-bound form. ATG10 has also been shown to interact with ATG12 in human embryonic kidney cells in the presence of ATG7. | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 0.5-1ug/ml | | Optimal dilutions to be determined by the researcher. | | Recommended Secondary Antibodies | I1903-30U: IgG, H&L, X-adsorbed (HRP) ShxRb | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, 0.02% sodium azide. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide corresponding to a 15 aa sequence from near the carboxy terminus of human ATG10. | | Specificity | Recognizes human Autophagy-related protein 10 (ATG10). Detects a band of approximately 33 kDa in SK-N-SH cell lysate. Species Crossreactivity: mouse, rat | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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