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You are here:Home » Antibodies » Antibodies-Autophagy » Anti -APG10L (Ubiquitin-like-conjugating Enzyme ATG10, Autophagy-related Protein 10, APG10-like, ATG10, PP12616)

Anti -APG10L (Ubiquitin-like-conjugating Enzyme ATG10, Autophagy-related Protein 10,
APG10-like, ATG10, PP12616)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes/vacuoles. The formation of autophagosomes involves a dynamic rearrangement of the membrane for which two ubiquitin-like modifications (the conjugation of APG12p and the modification of a soluble form of MAP-LC3 to a membrane-bound form) are essential. APG10L is an E2-like enzyme involved in two ubiquitin-like modifications essential for autophagosome formation, ATG12-ATG5 conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A), a homolog of yeast Apg8, to a membrane-bound form. The protein is ubiquitously expressed. Human APG10L gene is localized on chromosome 5q14.2.
Catalog #A2298-75G
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 2-6ug/ml
Immunohistochemistry (formalin fixed paraffin embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlBrain
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
FormSupplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa1-50 of human APG10L.
SpecificityRecognizes human APG10L. Species Crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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