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You are here:Home » Antibodies » Abs to Autophagy » Anti -APG5 (APG5 Autophagy 5-like, APG5-like, APG5L, Apoptosis-specific Protein, ASP, ATG5 Autophagy-related 5 Homolog, Autophagy Protein 5, hAPG5, Homolog of S Cerevisiae Autophagy 5)

Anti -APG5 (APG5 Autophagy 5-like, APG5-like, APG5L, Apoptosis-specific Protein, ASP, ATG5
Autophagy-related 5 Homolog, Autophagy Protein 5, hAPG5, Homolog of S Cerevisiae Autophagy
5)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Autophagy is an important mechanism for nonselective intracellular breakdown whereby cytosol and organelles are encapsulated in vesicles, which are then engulfed and digested by lytic vacuoles/lysosomes. ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. Conjugation to ATG12 is essential for autophagy, but is not required for association with isolation membrane. ATG5 also plays an important role in the apoptotic process. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.
Catalog #A2298-76
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionWestern Blot: 1:500
Immunohistochemistry: 2.5ug/ml
Optimal dilutions to be determined by the researcher.
Positive Control Mouse wildtype ES cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.1% sodium azide, 30% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenA synthetic peptide made to an N-terminal region (within residues 1-50) of the human ATG5 protein. (Swiss-Prot# Q9H1Y0)
SpecificityRecognizes ATG5. Selective for the full-length and calpain cleaved isoform proteins. The short isoform is missing aa1-79. The calpain cleaved form of ATG5 is missing aa195-275. Species sequence homology: human, rat, bovine, porcine, monkey and Xenopus, 100%; zebrafish, 94%
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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