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You are here:Home » Antibodies » Abs to Autophagy » Anti -Apg12 (Autophagy-related 12, 4931423H11Rik, A330058M13Rik, Atg12, Apg12L, APG12-like, Atg12L, Autophagy-related protein 12)

Anti -Apg12 (Autophagy-related 12, 4931423H11Rik, A330058M13Rik, Atg12, Apg12L,
APG12-like, Atg12L, Autophagy-related protein 12)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IC IF
Autophagy is a catabolic process for the autophagosomic- lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 (APG12) is covalently bound to Atg5 (APG5-like) and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin-E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8).
Catalog #A2298-76B
ApplicationsSuitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionsImmunofluorescence (IF-IC): 1:100
Western Blot: 1:1000
Immunoprecipitation: 1:25
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
PurityPurified by peptide affinity chromatography.
ImmunogenSynthetic peptide corresponding to residues near the amino terminus of mouse Apg12 (KLH).
SpecificityRecognizes endogenous levels of total free and Apg5-like bound mouse Apg12.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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