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You are here:Home » Antibodies » Abs to Autophagy » Anti -Apg12 (Autophagy-related 12, 4931423H11Rik, A330058M13Rik, Atg12, Apg12L, APG12-like, Atg12L, Autophagy-related protein 12)

Anti -Apg12 (Autophagy-related 12, 4931423H11Rik, A330058M13Rik, Atg12, Apg12L,
APG12-like, Atg12L, Autophagy-related protein 12)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified E B
Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. In yeast, autophagy, the delivery of cytoplasmic components to the lysosome/vacuole for degradation, requires a ubiquitin-like protein conjugation system, in which Apg12 is covalently bound to Apg12-Apg5 and Apg16.
Catalog #A2298-76E
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:1000-1:5000.
Western Blot: 1:2000.
Optimal dilutions to be determined by the researcher.
Positive Control Most yeast cell lysates can be used as a positive control without induction or stimulation.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceYeast
ConcentrationAs reported
Form0.15M Sodium Chloride, 0.02M Potassium Phosphate [pH 7] containing 0.01% sodium azide
PurityPurified
ImmunogenRecombinant full length protein (S. cerevisiae).
SpecificitySpecies Crossreactivity: Crossreacts with S. cerevisiae.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesAPG12, Autophagy-related Ubiquitin-like Modifier ATG12


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