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You are here:Home » Antibodies » Abs to Autophagy » Anti -APG5 (APG5 Autophagy 5-like, APG5-like, APG5L, Apoptosis-specific Protein, ASP, ATG5 Autophagy-related 5 Homolog, Autophagy Protein 5, hAPG5, Homolog of S Cerevisiae Autophagy 5) (HRP)

Anti -APG5 (APG5 Autophagy 5-like, APG5-like, APG5L, Apoptosis-specific Protein, ASP, ATG5
Autophagy-related 5 Homolog, Autophagy Protein 5, hAPG5, Homolog of S Cerevisiae Autophagy
5) (HRP)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Autophagy is an important mechanism for nonselective intracellular breakdown whereby cytosol and organelles are encapsulated in vesicles, which are then engulfed and digested by lytic vacuoles/lysosomes. ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. Conjugation to ATG12 is essential for autophagy, but is not required for association with isolation membrane. ATG5 also plays an important role in the apoptotic process. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.
Catalog #A2298-76H
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1:500
Immunohistochemistry: 2.5ug/ml
Optimal dilutions to be determined by researcher.
Positive ControlMouse wild-type ES cell lysate.
Storage and StabilityStore at 2-8°C. Do Not Freeze! Aliquots are stable for at least 3 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration1.17mg/ml
FormSupplied as a liquid in PBS, pH 7.4. Labeled with HRP.
PurityPurified by immunoaffinity chromatography.
ImmunogenA synthetic peptide made to an N-terminal region (aa1-50) of the human ATG5 protein.
SpecificitySelective for the full-length and calpain cleaved isoform proteins. The short isoform is missing aa1-79. The calpain cleaved form of ATG5 is missing aa195-275. Cellular Localization
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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