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You are here:Home » Antibodies » Abs to Autophagy » Anti -Atg16L1 (Autophagy-related Protein 16-1, ATG16 Autophagy Related 16-like 1, APG16-like 1, APG16 Autophagy 16-like 1)

Anti -Atg16L1 (Autophagy-related Protein 16-1, ATG16 Autophagy Related 16-like 1,
APG16-like 1, APG16 Autophagy 16-like 1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IH IF
Catalog #A2298-76T
APG16 L1 plays an essential role in autophagy. SUBUNIT Homooligomer. Interacts with ATG5. Part of either the minor and major complexes respectively composed of 4 sets of ATG12-ATG5 and ATG16L1 (400kD) or 8 sets of ATG12-ATG5 and ATG16L1 (800kD).
ApplicationsSuitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunofluorescence: 1:100-1:3000
Immunohistochemistry: 1:100-1:3000
Western Blot: 1:100-1:3000
Optimal dilutions to be determined by the researcher.
Cellular LocalizationCytoplasm. Preautophagosomal structure; preautophagosomal structure membrane; peripheral membrane protein. Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a lyophilized powder. Reconstitute in 100ul of sterile water. Centrifuge to remove any insoluble material.
PuritySerum
ImmunogenSynthetic peptide corresponding to the N-terminal of human ATG16-L1 protein conjugated to Blue Carrier Protein.
SpecificityRecognizes human ATG16 L1. Peptide Sequence Homology: mouse, rat, S. cerevisiae, Macaca mulatta (monkey) and Canis familiaris (dog).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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