| Aph-1 is a component of the gamma-secretase multiprotein complex, which consists of presenilin (PS), nicastrin, Aph-1 and Pen-2, which are all necessary for full proteolytic activity. g-Secretase is the proteolytic activity responsible for the cleavage of a series of integral membrane proteins, most notably the Amyloid Precursor Protein (APP) and Notch, but also including ErbB-4, E-Cadherin, LRP, and CD44.1 Aph-1 and Pen-2 are the limiting cellular factors that control presenilin expression, and thus, g-Secretase’s catalytic activity. Because g-Secretase is responsible for the release of amyloid peptide from APP, current research has centered around using g-Secretase as a potential Alzheimer’s disease drug target. |
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| Two mammalian homologues of Aph-1 have been identified: Aph-1a and Aph-1b, of which Aph-1a is more abundantly expressed than Aph-1b in human tissue samples. In addition, Aph-1a also occurs as two splice isoforms, long and short, that diverge at the C-terminus.3 |
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| Catalog # | A2298-77 |
| Applications | Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. |
| Recommended Dilution | Western Blot: 1-3ug/ml |
| Immunoprecipitation: 7-10ug/IP reaction |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~0.12mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Synthetic peptide derived from the C-terminal region of human Aph-1a. |
| Specificity | Recognizes human Aph-1a at ~29kD. A fragment of Aph-1a may also be observed at ~23kD. Recognizes only the long isoform of Aph-1a. Reactivity has been confirmed with human Aph-1a- transfected N2a mouse neuroblastoma lysates. Bands representing both endogenous and exogenous Aph-1a were observed. The non-transfected N2a cell lysates only showed endogenous Aph-1a expression. Species Crossreactivity: mouse. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
