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You are here:Home » Antibodies » Abs to Apoptosis » Anti -APIP (Probable Methylthioribulose-1-phosphate Dehydratase, MTRu-1-P Dehydratase, APAF1-interacting Protein, CGI-29)

Anti -APIP (Probable Methylthioribulose-1-phosphate Dehydratase, MTRu-1-P Dehydratase,
APAF1-interacting Protein, CGI-29)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B
The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1) (1,2). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis3. Recently, Cho et al (4) have identified a new Apaf-1 Interactiong Protein (APIP) also known as CG129 and MMRP19, as a negative regulator of ischemic injury. APIP competes with Caspase-9 binding site of Apaf1. APIP is predicted to code for a 204aa. An isoform of APIP, APIP2 encodes a 242aa protein, which is an alternative splicing variant differing in its N-terminus from APIP. APIP transcript is ubiquitously expressed in most adult tissue with high expression in skeletal muscle, heart, and kidney.
Catalog #A2298-79H3
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1-2ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlRecombinant protein
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No11E18
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant corresponding to full-length, His-tagged recombinant APIP protein.
SpecificityRecognizes human APIP.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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