Anti -APJ Receptor (APLNR, Apelin receptor, AGTRL1, angiotensin II receptor-like 1,
Angiotensin receptor-like 1, Apelin receptor, APJ, APJR, FLJ90771, G-protein coupled
receptor APJ, HG11, MGC45246)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E IH |
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| The apelin receptor (APJ receptor) has been identified as the endogenous receptor for the the peptide apelin. Previous to the identification of apelin, APJ was considered an orphan G protein coupled receptor. The apelin receptor (both by immunohistochemistry and in situ) has been localized to the CNS and is currently thought to play a role in stress response, feeding behavior and haemodynamic homeostasis. However, because of a broad distribution the apelin receptor and its endogenous ligand be involved in a wide range of biological process yet to be determined. | | | Catalog # | A2298-80K | | Applications | Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Immunohistochemistry: 1:10,000-1:50,000 | | Transfected COS-7 cells, plated on ploy-L-lysine coated glass coverslips, were fixed for 20 min with 4% paraformaldehyde in 0.1M PB, pH 7.4, rinsed with 0.1M TBS, pH 7.4, and preincubated for 30 min at RT with a blocking solution consisting of 5% normal goat serum, 2%BSA, AND 0.1% Triton X-100 in 0.1M TBS. Cells were incubated overnight at 4°C with anti-APJ peptide antiserum diluted to 1:10,000-1:50,000 in 0.1M TBS, pH 7.4 containing 1%NGS and 0.05% Triton X-100. After washing with TBS, cells were incubated for 1 hr at RT with appropriate secondary antibody, washed twice with TBS and mounted on glass slides with Aquamount. | | Optimal dilutions to be determined by the researcher. | | Control Peptides | A2298-80A, A2298-80B | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Host | Rabbit | | Source | Human | | Concentration | As reported | | Form | Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | (Cys)-TIAGHFRKERIEGLRK-amide | | Specificity | Recognizes human APJ Receptor (Apelin). | | Species Crossreactivity | Rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | APLNR, Apelin receptor, AGTRL1, angiotensin II receptor-like 1, Angiotensin receptor-like 1, Apelin receptor, APJ, APJR, FLJ90771, G-protein coupled receptor APJ, HG11, MGC45246 |
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Download Specifications for Anti -APJ Receptor (APLNR, Apelin receptor, AGTRL1, angiotensin II receptor-like 1, Angiotensin receptor-like 1, Apelin receptor, APJ, APJR, FLJ90771, G-protein coupled receptor APJ, HG11, MGC45246) |
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