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You are here:Home » Molecular Biology » MB-Enzymes, Cytidine Deaminase (APOBEC) » APOBEC3G, Recombinant, Human (CEM15, Apolipoprotein B mRNA-editing enzyme-catalytic polypeptide-like 3G)

APOBEC3G, Recombinant, Human (CEM15, Apolipoprotein B mRNA-editing enzyme-catalytic
polypeptide-like 3G)

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Specifications

Apolipoprotein B mRNA-editing enzyme-catalytic polypeptide-like 3G (APOBEC3G) is a host cellular protein with a broad antiviral activity. It inhibits infectivitiy of a wide variety of retroviruses by deaminating deoxycytidine (dC) into deoxyuridine (dU) in newly synthesized minus strand DNA, resulting in G-to-A hypermutation of the viral plus strand DNA.
Catalog #A2298-90
APOBEC3G, also known as CEM15 [1], is a host cellular protein which has a broad antiviral activity on a wide variety of retroviruses including HIV-1, other lentiviruses, and murine leukemia virus (MLV) [2-4]. The protein belongs to the Apobec superfamily of cytidine deaminases [5] and inhibits the infectivity of these viruses by being packaged into virions. During reverse transcription, it deaminates deoxycytidine (dC) into deoxyuridine (dU) in newly synthesized minus strand DNA, resulting in either G-to-A hypermutation of the viral plus strand DNA or degradation of dU-rich reverse transcripts [3,6-8], though several resent studies suggest cytidine deaminase adtivity is essential but not a sole determinant for antiviral activity of APOBEC3G. [7]. Most lentiviruses express an accessory protein called virion infectivity factor (Vif) which blocks the antiviral function of APOBEC3G by preventing its packaging into virions. Vif binds to APOBEC3G and induces its ubiquitination and subsequent degradation by the proteasome [9-13]. It has also been reported that APOBEC3G inhibits the replication of hepatitis B virus (HBV) without inducing G-to-A hypermutation [14]. This suggests that APOBEC3G has a broad antiviral activity not only on retroviruses but also on other viruses through different mechanisms from that on retroviruses.
AppiicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Storage and StabilityAliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months
SourceE. coli
PurityPurified by preparative SDS-PAGE, reduced, to 95% purity as determined by SDS-PAGE, reduced.
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 30% glycerol, 0.1% sarcosyl. No stabilizers or preservatives added.
Biological ActivityBinds HIV-1 vif as determined by ELISA and Western Blot; also complexes with native vif in HIV infected cells.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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