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You are here:Home » Antibodies » Abs to Enzymes, Cytidine Deaminase (APOBEC) » Anti -APOBEC3G (CEM15, Apolipoprotein B mRNA-editing enzyme-catalytic polypeptide-like 3G)

Anti -APOBEC3G (CEM15, Apolipoprotein B mRNA-editing enzyme-catalytic polypeptide-like 3G)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Highly Purified E
Apolipoprotein B mRNA-editing enzyme-catalytic polypeptide-like 3G (APOBEC3G) is a host cellular protein with a broad antiviral activity. It inhibits infectivitiy of a wide variety of retroviruses by deaminating deoxycytidine (dC) into deoxyuridine (dU) in newly synthesized minus strand DNA, resulting in G-to-A hypermutation of the viral plus strand DNA.
Catalog #A2298-90C
APOBEC3G, also known as CEM15 [1], is a host cellular protein which has a broad antiviral activity on a wide variety of retroviruses including HIV-1, other lentiviruses, and murine leukemia virus (MLV) [2-4]. The protein belongs to the Apobec superfamily of cytidine deaminases [5] and inhibits the infectivity of these viruses by being packaged into virions. During reverse transcription, it deaminates deoxycytidine (dC) into deoxyuridine (dU) in newly synthesized minus strand DNA, resulting in either G-to-A hypermutation of the viral plus strand DNA or degradation of dU-rich reverse transcripts [3,6-8], though several resent studies suggest cytidine deaminase adtivity is essential but not a sole determinant for antiviral activity of APOBEC3G. [7]. Most lentiviruses express an accessory protein called virion infectivity factor (Vif) which blocks the antiviral function of APOBEC3G by preventing its packaging into virions. Vif binds to APOBEC3G and induces its ubiquitination and subsequent degradation by the proteasome [9-13]. It has also been reported that APOBEC3G inhibits the replication of hepatitis B virus (HBV) without inducing G-to-A hypermutation [14]. This suggests that APOBEC3G has a broad antiviral activity not only on retroviruses but also on other viruses through different mechanisms from that on retroviruses.
Biological ActivityCEM15 biological activity neutralization assays pending.
ApplicationsA dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA may be performed at 10-100ng/ml of antibody depending on the amount of immobilized antigen in the assays. Western ELISA requires 2-5ug/ml antibody.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2. No stabilizers or preservatives added.
Purity95%
SpecificityBinds to native and recombinant human CEM15 in ELISA and Western ELISA.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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