| At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). The apo-lipoprotein used here is derived from human plasma by density gradient ultracentrifugation and HPLC. |
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| Catalog # | A2299-08V |
| Applications | Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | ELISA: 1:5000- 1:10000 |
| Immunohistochemistry: Paraffin sections |
| Western Blot: 1:500- 1:1000. |
| Optimal dilutions to be determined by the researcher. |
| Cellular Localization | Secreted |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Source | Mouse |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 0.15M sodium chloride, 0.02M potassium phosphate, pH 7, 0.01% sodium azide. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Full length native apoLipoprotein Type A-I (purified). |
| Specificity | This antibody reacts with mouse apoLipoprotein A-I and has negligible crossreactivity with Type A-II, B, C-I, C-II, C-III, E and J apoLipoproteins. Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
| Alternate names | APOA1/APOC3 fusion gene, Apolipoprotein A I precursor, Apolipoprotein of high density lipoprotein |
