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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E

Apolipoprotein AI promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein AI is the major protein component of high density lipoprotein (HDL) in the plasma. Synthesized in the liver and small intestine, it consists of two identical chains of 77aa; an 18aa signal peptide is removed co-translationally and a 6aa propeptide is cleaved post-translationally. Apolipoprotein A I is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in the Apolipoprotein A I gene are associated with HDL deficiency and Tangier disease. The therapeutic potential of apoA-I has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of apoA-I. The availability of recombinant normal apoA-I should facilitate further investigation into the potential usefulness of apoA-I in preventing atherosclerotic vascular diseases.
Catalog #	A2299-09A1
Applications	Suitable for use in ELISA. Other applications not tested
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Affinity Constant	5x10e6 L/mole (determined by RIA followed by Scatchard plot analysis)
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a,k
Clone No	9L39
Host	Mouse
Source	Human
Concentration	~5mg/ml
Form	Supplied as a liquid in 10mM phosphate, pH 7.4, 150mM sodium chloride, 0.1% sodium azide.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Human high density lipoprotein.
Specificity	Recognizes human Apolipoprotein AI. Does not cross react with Apo AII or Apo B in competitive RIA.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.