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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E

Apolipoprotein AI promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein AI is the major protein component of high density lipoprotein (HDL) in the plasma. Synthesized in the liver and small intestine, it consists of two identical chains of 77aa; an 18aa signal peptide is removed co-translationally and a 6aa propeptide is cleaved post-translationally. Apolipoprotein A I is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in the Apolipoprotein A I gene are associated with HDL deficiency and Tangier disease. The therapeutic potential of apoA-I has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of apoA-I. The availability of recombinant normal apoA-I should facilitate further investigation into the potential usefulness of apoA-I in preventing atherosclerotic vascular diseases.
Catalog #	A2299-12F
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions	Western Blot: 1:200-1:8000
	ELISA: 1:200-1:8000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Human
Concentration	As reported
Form	Supplied as a lyophilized powder from 50mM PBS, 0.1M sodium Chloride, pH 7.4, 10mg/ml BSA, 0.01% Thimerosal. Conjugated to HRP.
Purity	Purified by Apo AI-Sepharose affinity column chromatography.
Immunogen	Human Apo AI.
Specificity	Recognizes human Apo A-I. No cross-reaction with Apo AII, Apo B-100, Apo CI, Apo CII, Apo CIII, and Apo E.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.