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You are here:Home » Antibodies » Abs to Lipoproteins » Anti -Apolipoprotein A-I (Apo A-I)

Anti -Apolipoprotein A-I (Apo A-I)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E
Apolipoprotein A-1 (apo A-1) in human plasma is a major protein component of high density lipoprotein (HDL). The molecular mass is 24kD. Apo A-1 is a cofactor for enzymes involved in the normal turnover of cholesterol and is produced in the liver. Plasma concentration is 2.7-3.8mg/ml (1).
Catalog #A2299-26A
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recommended DilutionELISA: 1:500
Optimal dilutions to be determined by the researcher.
HybridomaX63-Ag8.653. Female CF1 x Balb/c mice immunized i.p. with immunogen adsorbed onto Al(OH)3.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No6F31
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 15mM sodium azide, before the addition of glycerol to 40%.
PurityPurified by Protein A/G affinity chromatography from culture supernatant.
ImmunogenApo A-1 isolated from human plasma.
SpecificityHas specificity for apo A-1 from human serum/plasma. No crossreactivity is seen with human apolipoprotein B. Reacts with apo A-1 from human serum/plasma when tested in sandwich ELISA, using a polyclonal antibody as coat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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