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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E

Apolipoprotein A-1 (apo A-1) in human plasma is a major protein component of high density lipoprotein (HDL). The molecular mass is 24kD. Apo A-1 is a cofactor for enzymes involved in the normal turnover of cholesterol and is produced in the liver. Plasma concentration is 2.7-3.8mg/ml (1).
Catalog #	A2299-26A
Applications	Suitable for use in ELISA. Other applications not tested.
Recommended Dilution	ELISA: 1:500
	Optimal dilutions to be determined by the researcher.
Hybridoma	X63-Ag8.653. Female CF1 x Balb/c mice immunized i.p. with immunogen adsorbed onto Al(OH)3.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	6F31
Host	Mouse
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide, before the addition of glycerol to 40%.
Purity	Purified by Protein A/G affinity chromatography from culture supernatant.
Immunogen	Apo A-1 isolated from human plasma.
Specificity	Has specificity for apo A-1 from human serum/plasma. No crossreactivity is seen with human apolipoprotein B. Reacts with apo A-1 from human serum/plasma when tested in sandwich ELISA, using a polyclonal antibody as coat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.