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Specifications

Clone Host Grade Applications Monoclonal Rat Affinity Purified E B

Apolipoprotein A2 (ApoA2) is a major protein component of serum HDL. It is produced by the liver and is involved in cholesteryl ester formation and cholesterol transport from tissues to the liver. Polymorphisms of ApoA2 are associated with disorders of cholesterol and fatty acid metabolism. ApoA2 can form disulfide-linked 17kD homodimers and heterodimers with other apolipoproteins.
Catalog #	A2299-31E
Applications	Suitable for use in Western Blot and ELISA. Other applications not tested.
Recommended Dilutions	Western Blot: 1-2ug/ml. The detection limit is 5ng/lane under reducing and non-reducing conditions.
	Direct ELISA: 0.5-1ug/ml. The detection limit is ~5ng/well.
	Optimal dilutions to be determined by the researcher.
Tested ELISA pair (see ref. 1)	Capture: A2299-32
	Detection: A2299-31E
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	7H205
Host	Rat
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Recombinant corresponding to aa24-100 of human ApoA2 expressed in E. coli (NP_001634).
Specificity	Recognizes human ApoA2. Species sequence homology: chimpanzee; 96%, bovine, equine, mouse, and rat; 48%-66%. Does not react with recombinant human ApoA1 or recombinant human APoE.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.