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You are here:Home » Antibodies » Abs to Lipoproteins » Anti -Apolipoprotein A-IV (APOA4, MGC142154, MGC142156)

Anti -Apolipoprotein A-IV (APOA4, MGC142154, MGC142156)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Catalog #A2299-36G
Recommended DilutionELISA: 1:32,000
Western Blot: 0.01-0.03ug/ml. ~ 45kD band observed in Human Duodenum lysates (calculated MW of 45.4kD according to NP_000473.2).
Optimal dilutions to be determined by the researcher.
Positive ControlHuman Duodenum lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris-saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide C-KEKESQDKTLSLP representing the C-Terminus (near) of the human protein (NP_000473.2.)
SpecificityRecognizes human Apolipoprotein A-IV.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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