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You are here:Home » Antibodies » Abs to Lipoproteins » Anti -Apolipoprotein B (Apo B)

Anti -Apolipoprotein B (Apo B)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E RIA
Apolipoprotein B plays an essential role in lipid transport and metabolism. Apo B may regulate cholesterol synthesis through its interaction with specific cell membrane receptors and by inhibition of HMG Co A reductase. This enzyme has been identified as the rate controlling enzyme in cholesterol biosynthesis. Apo B may be important in the genesis of atherosclerosis. Apo B is the major protein moiety of all lipoproteins other than high density lipoprotein (HDL) in plasma. Increased levels of apo B100 are associated with an increased risk of coronary artery disease.
Catalog #A2299-38B
ApplicationsSuitable for use in ELISA and RIA. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Affinity Constant1.0 x 10e8L/M Determined by RIA followed by Scatchard Plot analysis.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol 40-50%, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b,k
Clone No4A92
HostMouse
SourceHuman
Concentration~5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography. >90% determined by SDS-PAGE.
ImmunogenHuman Apolioprotein B
SpecificityRecognizes human Apolipoprotein B.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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