| Lipids (triglyceride (TG), cholesterol (Chol), phospholipids (PL), cholesteryl esters (CE)) are insoluble in plasma. These lipids combine with apolipoproteins to form lipoproteins. This process solubilizes lipids in plasma. Lipoproteins provide a transport system for lipids. They also play an important role in lipoprotein receptor recognition and regulation of certain enzymes in lipoprotein metabolism. Apolipoprotein B plays an essential role in lipid transport and metabolism. Apo B may regulate cholesterol synthesis through its interaction with specific cell membrane receptors and by inhibition of HMG Co A reductase. This enzyme has been identified as the rate controlling enzyme in cholesterol biosynthesis. Apo B may be important in the genesis of atherosclerosis. |
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| Catalog # | A2299-39 |
| Applications | Suitable for use in ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | ELISA: 1:2000-1:10000 |
| Western Blot: 1:200-1:1000 |
| Immunoprecipitation: 1:100 |
| Immunohistochemistry (Paraffin embedded tissue): 1:50-1:500 |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1904-28K: IgG, H&L (HRP) Pab RbxGt |
| I1904-28Q: IgG, H&L, X-Adsorbed (HRP) Pab RbxGt |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 0.125M sodium borate, 0.075M sodium chloride, 0.005M EDTA, pH 8.0, 0.01% sodium azide. No stabilizing proteins added. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Human Apolipoprotein Type B (isolated from human plasma by density gradient centrifugation followed by HPLC puri cation). |
| Specificity | Recognizes human apolipoprotein B. Assay by IEP resulted in a single precipitin arc against anti goat serum. Has negligible crossreactivity with Type A-I, A-II, C-I, C-II, C-III, E and J apolipoproteins. Typically less than 1% crossreactivity against other types of apolipoprotein was detected by ELISA against puri ed standards. Non-speci c crossreaction of anti-apolipoprotein antibodies with other human serum proteins is negligible. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
