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You are here:Home » Antibodies » Abs to Lipoproteins » Anti -Apolipoprotein B (Apo B) (Biotin)

Anti -Apolipoprotein B (Apo B) (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IP IH
Lipids (triglyceride (TG), cholesterol (Chol), phospholipids (PL), cholesteryl esters (CE)) are insoluble in plasma. These lipids combine with apolipoproteins to form lipoproteins. This process solubilizes lipids in plasma. Lipoproteins provide a transport system for lipids. They also play an important role in lipoprotein receptor recognition and regulation of certain enzymes in lipoprotein metabolism. Apolipoprotein B plays an essential role in lipid transport and metabolism. Apo B may regulate cholesterol synthesis through its interaction with specific cell membrane receptors and by inhibition of HMG Co A reductase. This enzyme has been identified as the rate controlling enzyme in cholesterol biosynthesis. Apo B may be important in the genesis of atherosclerosis. Apo B is the major protein moiety of all lipoproteins other than high density lipoprotein (HDL) in plasma. Increased levels of apo B100 are associated with an increased risk of coronary artery disease. Apo B exists in human plasma as two isoforms: apo B48 and apo B100. Apo B100 is the major physiological ligand for the LDL receptor. It contains 4536 amino acid residues. Apo B100 is the full length protein. Its gene has been mapped on the short arm of chromosome 2, with an approximate length of 43 kilobases and 29 exons. The LDL-binding domain of the molecule is proposed to be located between the aa3129-3532. Apo B100 is synthesized in the liver. It is required for the assembly of very low density lipoproteins (VLDL).
Catalog #A2299-39A
ApplicationsSuitable for use in ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:4000-1:20,000
Western Blot: 1:400-1:2000
Immunohistochemistry(Paraffin):1:100-1:1000
Immunoprecipitation: 1:200
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2. No preservative and no stabilizing protein added. Labeled with Biotin.
PurityPurified by immunoaffinity chromatography.
ImmunogenApolipoprotein Type B isolated from human plasma by density gradient centrifugation followed by HPLC puri cation.
SpecificityRecognizes human apolipoprotein B. Assay by IEP resulted in a single precipitin arc against anti goat serum. Has negligible crossreactivity with Type A-I, A-II, C-I, C-II, C-III, E and J apolipoproteins. Typically less than 1% crossreactivity against other types of apolipoprotein was detected by ELISA against puri ed standards. Non-speci c crossreaction of anti-apolipoprotein antibodies with other human serum proteins is negligible.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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