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Specifications

Clone Host Grade Applications Polyclonal Sheep Purified E

Lipids (triglyceride (TG), cholesterol (Chol), phospholipids (PL), cholesteryl esters (CE)) are insoluble in plasma. These lipids combine with apolipoproteins to form lipoproteins. This process solubilizes lipids in plasma. Lipoproteins provide a transport system for lipids. They also play an important role in lipoprotein receptor recognition and regulation of certain enzymes in lipoprotein metabolism. Apolipoprotein B plays an essential role in lipid transport and metabolism. Apo B may regulate cholesterol synthesis through its interaction with specific cell membrane receptors and by inhibition of HMG Co A reductase. This enzyme has been identified as the rate controlling enzyme in cholesterol biosynthesis. Apo B may be important in the genesis of atherosclerosis.
Catalog #	A2299-40E
Application	Suitable for use in ELISA. Other applications not tested.
Recommended Dilutions	ELISA: 1:1000. TMB can be used as a substrate at 1:800- 1:1600.
	Optimal dilutions to be determined by the researcher.
	Storage and Stability:
	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Sheep
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in 0.05% Proclin™300, 0.002% Methylisothiazolone and 0.002% Bromonitrodioxane. Labeled with HRP.
Purity	Purified.
Immunogen	Human apolipoprotein B purified from plasma.
Specificity	Recognizes human Apolipoprotein B. A single arc is detected when tested by IEP and 2D-IEP against human plasma.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.