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You are here:Home » Antibodies » Abs to Lipoproteins » Anti -Apolipoprotein B (Apo B)

Anti -Apolipoprotein B (Apo B)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Apolipoprotein B (apo B) in human plasma is a major protein of low density lipoproteins (LDL). The molecular mass is 260kD. Apo B binds to specific receptors on cell membranes and is involved in removal of LDL and very low density lipoprotein (VLDL) cholesterol from circulation.
Catalog #A2299-41
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:16,000
Optimal dilutions to be determined by the researcher.
Culture mediumRPMI with 10% fetal calf serum
HybridomaX63-Ag8.653. Female CF1 x Balb/c mice immunized i.p. with immunogen adsorbed onto Al(OH)3.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b,k
Clone No6F33
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 15mM sodium azide, before the addition of glycerol to 40%.
PurityPurified by Protein A/G affinity chromatography from culture supernatant.
ImmunogenHuman LDL.
SpecificityRecognizes Apo B in human serum/plasma. No crossreactivity is seen with human apolipoprotein A-1. Crossreacts with LDL. Epitope differs from that of clones 6F34 and 6F35.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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