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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E

Apolipoprotein B (apo B) in human plasma is a major protein of low density lipoproteins (LDL). The molecular mass is 260kD. Apo B binds to specific receptors on cell membranes and is involved in removal of LDL and very low density lipoprotein (VLDL) cholesterol from circulation.
Catalog #	A2299-41B
Applications	Suitable for use in ELISA. Other applications not tested.
Recommended Dilutions	ELISA: 1:18000
	Optimal dilutions to be determined by the researcher.
Culture Medium	RPMI 1640 with 2-10% fetal calf serum
Hybridoma	X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	6F35
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Purity	Purified by Protein A/G affinity chromatography.
Immunogen	Human LDL
Specificity	Recognizes human Apolipoprotein B from human serum/plasma. Does not crossreact with human apolipoprotein A-1. Epitope differs from that of clones 6F33 and 6F34.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.