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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B IP

Apolipoprotein B consists of a single polypeptide chain with a molecular weight of 549kD and is mostly synthesized in the liver. It is a major apolipoprotein of very low density, intermediate density and low density lipoproteins (LDL) as well as being a major component of lipoprotein (a). Apolipoprotein B is a ligand for the LDL receptor and elevated levels are associated with premature atherosclerosis. Normal plasma apolipoprotein B levels are around 800mg/L.
Catalog #	A2299-46E
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	ELISA: 1:2,000-1:10,000
	Western Blot: 1:2,000-1:10,000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Host	Goat
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in borate buffered saline, pH 8.0, 0.01% sodium azide.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Apolipoprotein B isolated from human plasma by density gradient centrifugation followed by HPLC purification.
Specificity	Recognizes human Apolipoprotein B (Apo B100 and Apo B48).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.