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You are here:Home » Antibodies » Abs to Lipoproteins » Anti -Apolipoprotein B (Apo B, APOB, Apolipoprotein B-100, Apo B-100)

Anti -Apolipoprotein B (Apo B, APOB, Apolipoprotein B-100, Apo B-100)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IP IH
Apolipoprotein B consists of a single polypeptide chain with a molecular weight of 549kD and is mostly synthesised in the liver. It is a major apolipoprotein of very low density, intermediate density and low density lipoproteins (LDL) as well as being a major component of lipoprotein (a). Apolipoprotein B is a ligand for the LDL receptor and elevated levels are associated with premature atherosclerosis. Normal plasma apolipoprotein B levels are around 800 mg/L.
Catalog #A2299-46H
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 0.5-1ug/ml
Western Blot: 0.5-1ug/ml
Immunoprecipitation: 5-10ug/ml
Immunohistochemistry: 5-10ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.01% thimerosal, 50% glycerol.
PurityPurified by affinity chromatography.
ImmunogenApolipoprotein purified from human plasma.
SpecificityRecognizes human ApoLipoprotein. It shows negligible crossreactivity with type A-I, A-II, C-I, C-II, C-III, E, and J apoLipoproteins (< 1%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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