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You are here:Home » Antibodies » Abs to Lipoproteins » Anti -Apolipoprotein B (Apo B, ApoB, ApoB Protein, Apolipoprotein B-48, ApoB-48, Apolipoprotein B-100, ApoB-100, Apo B-100, FLDB, LDLCQ4)

Anti -Apolipoprotein B (Apo B, ApoB, ApoB Protein, Apolipoprotein B-48, ApoB-48,
Apolipoprotein B-100, ApoB-100, Apo B-100, FLDB, LDLCQ4)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Highly Purified E IH
Apolipoprotein B plays an essential role in lipid transport and metabolism. Apo B may regulate cholesterol synthesis through its interaction with specific cell membrane receptors and by inhibition of HMG Co A reductase. This enzyme has been identified as the rate controlling enzyme in cholesterol biosynthesis. Apo B may be important in the genesis of atherosclerosis. Apo B is the major protein moiety of all lipoproteins other than high density lipoprotein (HDL) in plasma. Apo B exists in human plasma as two isoforms: apo B48 and apo B100. Apo B100 is the major physiological ligand for the LDL receptor. It contains 4536aa residues. Apo B100 is the full length protein. Its gene has been mapped on the short arm of chromosome 2, with an ~length of 43kb and 29 exons. Apo B100 is synthesised in the liver. It is required for the assembly of very low density lipoproteins (VLDL). It does not interchange between lipoprotein particles, as do the other lipoproteins. It is found in IDL and LDL particles after the removal of the apolipoproteins A, E and C. Apo B48 is present in chylomicrons and chylomicron remnants. It plays an essential role in the intestinal absorption of dietary fats. Apo B48 is synthesized in the small intestine. It comprises the N-terminal 48% of apo B100. Apo B48 is the degraded protein. Apo B48 lacks the LDL receptor-binding domain of Apo B100. It is produced due to post-transscriptional Apo B100 mRNA editing at codon 2153, which creates a stop codon in the intestine instead of a glutamine in the liver.
Catalog #A2299-52J
ApplicationsSuitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:1000
Immunohistochemistry (Frozen): 1:10
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2000
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder from 10mM sodium phosphate, pH 8.0, 100mM sodium chloride, 0.1% mannitol, 1mM sodium azide. Reconstitute with 1ml sterile ddH2O.
PurityPurified by sodium sulfate and DEAE-cellulose chromatography.
SpecificityRecognizes human Apolipoprotein B (Apo-B) from human plasma. Specific for fragment T2, the carboxyterminal thrombolytic peptide of human Apo-B. No crossreactions with other human plasma proteins were detected.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesApolipoprotein B (including Ag(x) Antigen)


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