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You are here:Home » Antibodies » Abs to Lipoproteins » Anti -APOB (Apolipoprotein B-100, Apo B-100)

Anti -APOB (Apolipoprotein B-100, Apo B-100)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IP IH
Apolipoprotein B (ApoB) is the main apolipoprotein of chylomicrons and low density lipoproteins (LDL). The protein occurs in the plasma in 2 main isoforms, apoB-48 and apoB-100. The first is synthesized exclusively by the gut, the second by the liver. The intestinal (B-48) and hepatic (B-100) forms of apoB are coded by a single gene and by a single mRNA transcript larger than 16kb. The 2 proteins share a common amino terminal sequence. In the ApoB-100 isoform the precursor has 4563aa, and the mature apoB-100 has 4536aa residues. Mature, circulating B-48 is homologous over its entire length (estimated to be between 2130 and 2144aa residues) with the amino-terminal portion of B-100 and contains no sequence from the carboxyl end of B-100. From structural studies, it is thought that apoB-48 represents the amino-terminal 47% of apoB-100 and that the carboxyl terminus of apoB-48 is in the vicinity of residue 2151 of apoB-100. Apolipoprotein B-48 may be the product of an intestinal mRNA with an in-frame UAA stop codon resulting from a C-to-U change in the codon CAA encoding Gln(2153) in apoB-100 mRNA. Since only the sequence that codes B-100 is present in genomic DNA, this presents the possibility of an organ-specific introduction of a stop codon to an mRNA and the change from CAA to UAA of codon 2153 of the message as a unique RNA editing process.
Catalog #A2299-52M
ApplicationsSuitable for use in ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:2000-1:10,000
Western Blot: 1:2000-1:10,000
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostGoat
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in 0.125M sodium borate, 0.075M sodium chloride, 0.005M EDTA, pH 8.0, 0.01% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenAPOB isolated from human plasma by density gradient centrifugation followed by HPLC purification.
SpecificityRecognizes human APOB. Negligible cross-reactivity with Type A-I, A-II, C-I, C-II, C-III, E and J Apolipoproteins and other human serum proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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