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You are here:Home » Antibodies » Abs to Lipoproteins » Anti -Apolipoprotein B, APOB (including Ag(x) antigen)

Anti -Apolipoprotein B, APOB (including Ag(x) antigen)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels.
Catalog #A2299-53
ApplicationsSuitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended Dilutions
Peptide ELISA Titer: 1:32,000
Western Blot: 0.3-1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
PurityPurified by peptide affinity chromatography.
ImmunogenSynthetic peptide corresponding to C-TDLHLRYQKDKK, from the internal region of human Apolipoprotein B (NP_000375.2).
SpecificityRecognizes human Apolipoprotein B.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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