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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B IP IH

Apolipoproteins which are present in human plasma transport circulating lipids throughout the body. Abnormal concentrations of these proteins in the blood have been correlated with atherosclerotic disorders including coronary heart disease and cerebrovascular disease. Approximately 50% of the HDL particle is protein, 70% Apo AI and 20% Apo AII. LDL contains approximately 20% protein, 95% of which is Apo B100. Apo C's, Apo B and Apo E are found in VLDL particles, accounting for approximately 10% of this particle.
Catalog #	A2299-63
Applications	Suitable for use in ELISA, Immunoprecipitation, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 1:5,000-1:10,000
	ELISA: 1:5,000-1:10,000
	Immunohistochemistry (paraffin): 1:50-1:200.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Human
Concentration	~1mg/ml (UV 280)
Form	Supplied as a liquid in 0.125M sodium borate, 0.075M sodium chloride, 0.005M EDTA, pH 8.0, 0.01% sodium azide. No stabilizing proteins added.
Purity	Purified by affinity chromatography.
Immunogen	Synthetic peptide full length ApoLipoprotein Type C-I.
Specificity	Recognizes human apoLipoprotein C-I and has negligible crossreactivity with Type A-I, A-II, B, C-II, C-III, E and J apoLipoproteins. Non-speci c crossreaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible. (Less than 1% crossreactivity against other types of apoLipoprotein was detected by ELISA against puri ed standards.)
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.