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You are here:Home » Antibodies » Abs to Lipoproteins » Anti -Apolipoprotein C-II (Apo C-II) (Biotin)

Anti -Apolipoprotein C-II (Apo C-II) (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified B
Apolipoprotein C2 (ApoC2/II) is a 8-9kD, secreted member of the Apolipoprotein C2 family of proteins. It is produced by hepatocytes and represents a major component of VLDL particles. It activates lipoprotein lipase and may self-associate to form amyloid-type fibrils. The human ApoC2 precursor is 101 amino acids (aa) in length. It contains a 22 amino acid (aa) signal sequence, followed by a 79 aa ProApoC2 that contains a lipid-binding region (aa 43-51) and an enzyme interaction site (aa 55-78). ProApoC2 represents > 90% of circulating ApoC2. In human, limited proteolytic processing occurs with removal of the six aa prosegment (aa 23-28). This does not affect activity. Human ProApoC2 is 59% aa identical to mouse and dog ProApoC2, respectively.
Catalog #A2299-64B
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.25ug/mL
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder in PBS containing 50ug of bovine serum albumin (BSA). Label with (Biotin) Reconstitute with 200ul 40-50% glycerol, TBS, 0.1% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenE. coli-derived recombinant human Apolipoprotein C2. (aa 23 - 101)
SpecificityRecognizes human Apolipoprotein C2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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