| At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). |
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| Catalog # | A2299-68A |
| Applications | Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation and ELISA. Other applications not tested. |
| Recommended Dilutions | Western Blot: 1:5000-1:10,000 |
| Immunohistochemistry (Paraffin): 1:50-1:200 |
| ELISA: 1:5000-1:10,000. Using 1ug of Apo C-III in standard sandwich. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 0.125M sodium borate, 0.075M sodium chloride, pH 8.0, 0.005M EDTA, 0.01% sodium azide. |
| Purity | Purified by affinity chromatography. |
| Immunogen | Synthetic peptide corresponding to Apolipoprotein C-III. |
| Specificity | Recognizes human Apolipoprotein C-III. Negligible crossreactivity to Apo A-I, A-II, B, C-I, C-II, E and J by ELISA. Negligible non-specific crossreaction with other human serum proteins. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
