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You are here:Home » Antibodies » Abs to Lipoproteins » Anti -Apolipoprotein CII (Apo CII)

Anti -Apolipoprotein CII (Apo CII)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). Apolipoprotein C-II (apoCII) is in found in chylomicrons (large lipoprotein particles absorbed from the gastrointestinal tract) and VLDL (large lipoproteins that are broken down to eventually form LDL). ApoCII activates the enzyme lipoprotein lipase, which hydrolyzes triglycerides and thus provides free fatty acids for cells.
Catalog #A2299-68E
ApplicationsSuitable for use in ELISA, Western Blot, Enzyme Conjugation, or Biotinylation. Other applications not tested.
Recommended DilutionELISA: 1:2,000-1:8,000
Immunoblot: 1:2,000-1:8,000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 10mM PBS, 0.15M sodium chloride, 1mM EDTA, pH 7.2, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenHuman Apolipoprotein CII.
SpecificityRecognizes human Apolipoprotein CII (Apo CII).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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