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Specifications

Clone Host Grade Applications Monoclonal Mouse Supernatant B IH

Apolipoprotein D is a glycoprotein involved with the plasma lipid transport system. It may act in cholesterol esterification and the transport of products in this reaction. It is found widely in tissues including axillary apocrine glands, adrenal cortex, corpus luteum, nerves, pituitary, testis, cerebellum and renal tubules. It has been reported to be present in malignant prostatic glands, but absent from normal glands.
Catalog #	A2299-72C
Applications	Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 1:500-1:1000
	Immunohistochemistry (Paraffin): 1:40-1:80 for 1 hour at RT
	Optimal dilutions to be determined by the researcher.
Positive Control
	Western Blot: ZR75 cell line
	Immunohistochemistry: Testis, Granular staining pattern of apolipoprotein D-containing lipid droplets
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	36C6
Host	Mouse
Concentration	Not determined
Form	Supplied as a lyophilized powder in 15mM sodium azide. Reconstitute with 1ml dH2O.
Purity	Supernatant
Immunogen	Recombinant protein representing the mature full-length apolipoprotein D molecule.
Specificity	Recognizes Apolipoprotein D. Species Crossreactivity: Human.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.