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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B

Lipoproteins are responsible for carrying cholesterol and other fats through the bloodstream as little packages and are essential for the normal breakdown of these molecules. In particular, apolipoprotein E is a major component of specific lipoproteins called very low-density lipoproteins (VLDL). A major function of VLDLs is to remove excess cholesterol from the blood and carry it to the liver for processing. Maintaining normal levels of cholesterol is essential for the prevention of cardiovascular diseases, including heart attacks and strokes. Apolipoprotein E is a type of lipoprotein. Apolipoprotein E is associated with several cardiovascular disorders. There are three isoforms (slightly different forms) of the ApoE lipoprotein. These three isoforms are known as ApoE2, E3 and E4. ApoE2 seems to be one of several genetic factors that plays a part in increased risk of heart attacks and strokes and ApoE4 is a risk factor for coronary artery disease. As well, ApoE4 has been been suggested to play a role in type 2 Alzheimer disease.
Catalog #	A2299-74D
Applications	Suitable for use in ELISA, Western Blotting. Other applications not tested.
Recommended Dilution	Western Blotting: 2ug/ml. A band is seen at ~36kD representing the ApoE protein.
	Optimal dilutions to be determined by the researcher.
Localization	Secreted
Positive Controls	Human brain and liver lysate
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	8B65
Host	Mouse
Source	Human
Concentration	~0.1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Synthetic peptide corresponding to a region between aa140 and 160 of purified human ApoE.
Specificity	Recognizes human Apolipoprotein E. Does not appear to react with rat brain tissue.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.