You are here:Home
»
Kits and Assays
»
Abs to Enzymes, Cytochrome
»
Apoptosis Assay, Cytochrome C Releasing BioAssay™ Kit
Apoptosis Assay, Cytochrome C Releasing BioAssay™ Kit
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Cytochrome c (cyt-c) plays an important role in apoptosis. The protein is located in the space between the inner and outer mitochondrial membranes. An apoptotic stimulus triggers the release of cytochrome c from the mitochondria into cytosol where it binds to Apaf-1. The cytochrome c/Apaf-1 complex activates caspase-9, which then activates caspase-3 and other downstream caspases. Cytochrome c Releasing Apoptosis Assay Kit provides an effective means for detecting cytochrome c translocation from mitochondria into cytosol during apoptosis. The kit provides unique formulations of reagents to isolate a highly enriched mitochondria fraction from cytosol. The procedure is so simple and easy to perform, no ultracentrifugation is required and no toxic chemicals are involved. Cytochrome c releasing from mitochondria into cytosol is then determined by Western blotting using the cytochrome c antibody provided in the kit. | | | Catalog # | A2299-90 | | Kit Contents (100 assays) | Volume Cap Storage | | A2299-90A: Mitochondria Extraction Buffer 10ml NM 4°C | | A2299-90B: 5X Cytosol Extraction Buffer 20ml WM 4°C | | A2299-90C: DTT (1M) 110ul Blue -20°C | | A2299-90D: 500X Protease Inhibitor Cocktail | | (Add 250ml of DMSO before use. 1 vial Red -20°C | | A2299-90E: Cytochrome c Antibody | | (0.2mg/ml) 500ul Green -20°C | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
External Links
|
