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Anti -Aprotinin (Pancreatic trypsin inhibitor) (Biotin)
Anti -Aprotinin (Pancreatic trypsin inhibitor) (Biotin)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E |
|
| Aprotinin, also called bovine pancreatic trypsin inhibitor, is a single chain globular Kunitz-type serine proteinase inhibitor found in bovine pancreas, parotid gland and lung. It forms stable, enzymatically inactive complexes with trypsin, chymotrypsin, plasmin and kallikrein. Aprotinin has been used therapeutically as an antifibrinolytic agent under the name Trasylol (Bayer) to reduce blood loss during cardiopulmonary bypass surgery but this use has been associated with an increased frequency of acute renal injury (1). | | | Catalog # | A2300-01B | | Applications | Suitable for use in ELISA. Other applications not tested. | | Recommended Dilutions | ELISA: 1:20,000 | | Optimal dilutions to be determined by the researcher. | | Matched Pair | Capture: | | A2300-01C (Samples concentrations: 0.6-20ug/ml) | | A2300-01 (Samples concentrations: 4-250ug/ml) PBS-buffers are unsuitable for these assays and 0.2M glycine sodium buffer, pH 8.8, is recommended. | | Detection: | | A2300-01B | | Culture Medium | RMPI 1640 with 2-10% fetal calf serum | | Hybridoma | Sp2mIL6 myeloma cells with spleen cells from NMRI x Balb/c mice. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1k | | Clone No | 9A509 | | Host | Mouse | | Source | Bovine | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS pH 7.4, 15mM sodium azide. Labeled with Biotin. | | Purity | Purified by Protein A/G affinity chromatography. | | Immunogen | Aprotinin from bovine lung adsorbed onto aluminum hydroxide gel. | | Specificity | Recognizes bovine aprotinin immobilized on a solid phase or free in solution. Epitope is different from A2300-01 and A2300-01C. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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