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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B

APS is an SH2 and PH domain-containing adaptor protein closely related to Lnk and SH2-B (1). APS was identified as a substrate for many receptor tyrosine kinases including TrkA, insulin receptor, c-Kit and PDGF receptor (2). Tyrosine phosphorylation of APS provides docking sites for downstream signalingcomponents, mediating diverse signaling pathways. APS plays quite different roles in RTK signaling. Overexpression of APS has been shown to inhibit PDGF-induced mitogenicity, which may result from APS/c-Cbl-mediated PDGF receptor degradation (3). However, APS promotes enhanced mitogenicity in response to insulin stimulation (4). The striking difference in APS-mediated signaling between the different RTKs could lie in the mode of interaction with the respective receptor.
Catalog #	A2500
Applications	Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 1:1000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	As reported
Form	Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150 mM NaCl, 100ug/ml BSA and 50% glycerol.
Purity	Purified by Protein A and immunoaffinity chromatography.
Immunogen	Synthetic peptide (KLH-coupled) corresponding to residues near the
amino-terminus of human APS.
Specificity	Detects endogenous levels of total APS. Does not crossreact with other adaptor/docking proteins. Species Crossreactivity: human and mouse
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.