| Aquaporin 1 is a 28kD intergral membrane protein which was originally identified in red blood cells and the kidney. AQP1 is also expressd by the choroid plexus and various other tissues. The glycosylated forms of AQP1 range between 40-60kD in mass. |
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| Catalog # | A2999-50C |
| Applications | Suitable for use in Immunohistochemistry, ELISA, Immunoprecipitation and Western Blot. Other applications not tested. |
| Recommended Dilution | Immunohistochemistry (Frozen and paraffin): 1:500-1:1000, Note: Antigen retrieval using heat treatment prior to staining of paraffin section is required. Sodium citrate buffer pH 6.0 is recommended for this purpose. |
| ELISA: 1:1000-1:20,000 |
| Immunoprecipitation: 1:1000-1:10,000 |
| Western Blot: 1:1000-1:5000 |
| Optimal working dilutions to be determined by researcher. |
| Recommended Secondary Reagents | I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo |
| Recommended Positive Control | Kidney |
| Hybridoma | Spleen cells from immunized Balb/c mice were fused with the cells of Sp-2/0 Ag14 mouse myeloma cell line. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3H551 |
| Host | Mouse |
| Source | Rat |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 0.09% sodium azide, 0.1% BSA. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Synthetic peptide corresponding to aa249-269 of rat aquaporin 1. |
| Specificity | Recognizes an epitope within the cytoplasmic domain of the water-specific channel rat aquaporin 1 (also known as AQP1 or CHIP-28). Species Crossreactivity: mouse. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
