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You are here:Home » Antibodies » Abs to Aquaporin » Anti -Aquaporin 1 (AQP1, CHIP-28, Channel-forming Integral Protein)

Anti -Aquaporin 1 (AQP1, CHIP-28, Channel-forming Integral Protein)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH
Aquaporin 1 is a 28kD intergral membrane protein which was originally identified in red blood cells and the kidney. AQP1 is also expressd by the choroid plexus and various other tissues. The glycosylated forms of AQP1 range between 40-60kD in mass.
Catalog #A2999-50C
ApplicationsSuitable for use in Immunohistochemistry, ELISA, Immunoprecipitation and Western Blot. Other applications not tested.
Recommended DilutionImmunohistochemistry (Frozen and paraffin): 1:500-1:1000, Note: Antigen retrieval using heat treatment prior to staining of paraffin section is required. Sodium citrate buffer pH 6.0 is recommended for this purpose.
ELISA: 1:1000-1:20,000
Immunoprecipitation: 1:1000-1:10,000
Western Blot: 1:1000-1:5000
Optimal working dilutions to be determined by researcher.
Recommended Secondary ReagentsI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
Recommended Positive ControlKidney
HybridomaSpleen cells from immunized Balb/c mice were fused with the cells of Sp-2/0 Ag14 mouse myeloma cell line.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H551
HostMouse
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in 0.09% sodium azide, 0.1% BSA.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa249-269 of rat aquaporin 1.
SpecificityRecognizes an epitope within the cytoplasmic domain of the water-specific channel rat aquaporin 1 (also known as AQP1 or CHIP-28). Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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