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You are here:Home » Antibodies » Abs to Aquaporin » Anti -Aquaporin 1 (AQP1, CHIP-28, Channel-forming Integral Protein)

Anti -Aquaporin 1 (AQP1, CHIP-28, Channel-forming Integral Protein)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH IF
Aquaporin 1 is a 28kD integral protein thought at first to be a breakdown product of the Rh polypeptide but was later shown to be a unique molecule that is abundant in erythrocytes and renal tubules. AQP1 is also expressed by the choroid plexus and various other tissues. It forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
Catalog #A3000-01B
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence. Other applications not tested.
Recommended DilutionsELISA: 1:1000-1:20,000
Western Blot: 1:1000-1:5000; Predicted molecular weight 32.3kD.
Immunoprecipitation: 1:1000
Immunohistochemistry (Frozen): 1:500-1:1000
Immunohistochemistry (Paraffin): 1:500-1:1000. Heat mediated antigen retrieval is required for paraffin sections.
Immunofluorescence (IC): 1:10-1:2000
Optimal dilutions to be determined by the researcher.
Positive ControlKidney
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No8J300
HostMouse
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa249-269 of rat Aquaporin 1. Cellular Localization: Plasma membrane
SpecificityRecognizes an epitope within the cytoplasmic domain of the water-specific channel of rat Aquaporin 1.
Species CrossreactivityHuman, mouse, rabbit and zebrafish
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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