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You are here:Home » Molecular Biology » MB-Aquaporin » Aquaporin 1, Rat, Control Peptide (AQP1, CHIP, CHIP28)

Aquaporin 1, Rat, Control Peptide (AQP1, CHIP, CHIP28)

Pricing

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Specifications

Control Peptide for A2999 (affinity purified antibody) and A3000-03B (antiserum).
Catalog #A3000-03D
Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein “aquaporin”. Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. Aquaporin-0 or MIP26 (major intrinsic protein 26kD), and Aquaporin-1 (purified from red cells) also called CHIP-28 (channel forming integral protein, 28kD; 268aa; gene locus 7p14) has been the foundation of the growing family of aquaporins. The lens specific Aquaporin-0 represents up to 80% of total lens membrane protein.
ApplicationsSuitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested.
Recommended DilutionsELISA: 50-100ng control peptide/well
Antibody Blocking: 5-10ug per 1ug A2999 (affinity purified antibody) and 1ul A3000-03B (antiserum).
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceRat
PurityHighly purified
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.5.
SpecificitySynthetic peptide 19aa sequence corresponding to the C-terminal, cytoplasmic domain of rat AQP1. No significant homology to any other known eukaryotic protein. Species Sequence Homology: mouse, rat, human, and bovine 100%.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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