Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Aquaporin » Anti -Aquaporin 3 (AQP3, GLIP, Glycerol-transporting Integral Protein)

Anti -Aquaporin 3 (AQP3, GLIP, Glycerol-transporting Integral Protein)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH IF
Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein “aquaporin”. Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. Aquaporin-0 or MIP26 (major intrinsic protein 26kD), and Aquaporin-1 (purified from red cells) also called CHIP-28 (channel forming integral protein, 28kD; 268aa; gene locus 7p14) has been the foundation of the growing family of aquaporins. The lens specific Aquaporin-0 represents up to 80% of total lens membrane protein. Defects in MIP26 are a cause of autosomal dominant cataract. The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP. The lens opacity mutation (LOP) is an AA substitution that inhibits targeting of MIP to the cell membrane. Human Aquaporin-0 is a 263 amino acid transmembrane protein belonging to the MIP family. Aquaporin families of proteins are predicted to contain six transmembrane domains. The N and C-terminus are predicted to be cytoplasmic.
Catalog #A3000-09
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications not tested.
Recommended DilutionWestern Blot: 1-10ug/ml.
ELISA: 1:1000.
Immunohistochemistry: 2-10ug/ml. See references.
Optimal dilutions to be determined by the researcher.
Control Peptide A3000-11G
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 0.1% BSA. Reconstitute with 100ul sterile PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to 15aa near the C-terminal, cytoplasmic domainof rat AQP3, conjugated to KLH.
SpecificityRecognizes rat Aquaporin 3. Species Sequence Homology: mouse; 92%, human; 85%.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links