| Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporins". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. AQP4 (MIWC, mercury-insensitive water channel) is expressed in brain and in other tissues as well. |
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| Catalog # | A3000-14 |
| Applications | Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. |
| Recommended Dilutions | Immunohistochemistry: 2-10ug/ml on paraformaldehyde-fixed or 50% methanol/50% acetone-fixed sections of tissues. |
| Western Blot: 1-10ug/ml using Chemiluminescence technique. Note: Recommend the use of 0.5-1% milk in all primary/secondary antibody-enzyme conjugate incubations in order to suppress non-specific bands. |
| ELISA: 1:100,000. A3000-18 Control peptide can be used to coat ELISA plates at 50-100ng/well. |
| Optimal dilutions to be determined by researcher. |
| Control Peptide | A3000-18: Aquaporin 4, Rat Control Peptide |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Rat |
| Concentration | ~1mg/ml |
| Form | Supplied as a lyophilized powder from PBS, pH 7.4, 0.1% BSA. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Synthetic peptide corresponding to17aa C-terminal, aa280-296 of rat AQP4 (KLH coupled via cysteine amino terminal). Cellular Localization: Multi-pass membrane protein. |
| Specificity | Recognizes rat Aquaporin 4 (AQ, WCH4; MIWC). Immunizing sequence is unique to AQP4. Species Sequence Homology |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
