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You are here:Home » Antibodies » Abs to Aquaporin » Anti -Aquaporin 4 (AQP4, WCH4, MIWC, Mercury-insensitive Water Channel)

Anti -Aquaporin 4 (AQP4, WCH4, MIWC, Mercury-insensitive Water Channel)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH
Aquaporin 4 is located in various tissues including the gastro intestinal tract, kidney and brain.
Catalog #A3000-14A
ApplicationsSuitable for use in Immunohistochemistry, ELISA, Immunoprecipitation and Western Blot. Other applications not tested.
Recommended DilutionImmunohistochemistry(frozen and paraffin): 1:50 Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
ELISA: 1:500-1:10,000
Immunoprecipitation: 1:500-1:5000
Western Blot: 1:500-1:5000
Optimal working dilutions to be determined by researcher.
Recommended Secondary ReagentsI1904-65W: IgG, F(abí)2 (HRP) Pab RbxMo
I1904-66C: IgG, F(abí)2, X-Adsorbed (HRP) Pab RbxMo
Recommended Positive Control TissueKidney
HybridomaSpleen cells from immunized Balb/c mice were fused with the cells of Sp-2/0 Ag14 mouse myeloma cell line.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H552
HostMouse
Concentration~0.5mg/ml
FormSupplied as a liquid with 0.1% BSA., 0.09% sodium azide, before addition of glycerol to 40%
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide corresponding to amino acids 301-318 of rat aquaporin 4.
SpecificityRecognizes an epitope within the cytoplasmic domain of the water-specific channel of human aquaporin 4 (AQP4). Does not react with mouse or rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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