| Aquaporin 4 is located in various tissues including the gastro intestinal tract, kidney and brain. |
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| Catalog # | A3000-14A |
| Applications | Suitable for use in Immunohistochemistry, ELISA, Immunoprecipitation and Western Blot. Other applications not tested. |
| Recommended Dilution | Immunohistochemistry(frozen and paraffin): 1:50 Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. |
| ELISA: 1:500-1:10,000 |
| Immunoprecipitation: 1:500-1:5000 |
| Western Blot: 1:500-1:5000 |
| Optimal working dilutions to be determined by researcher. |
| Recommended Secondary Reagents | I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo |
| I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo |
| Recommended Positive Control Tissue | Kidney |
| Hybridoma | Spleen cells from immunized Balb/c mice were fused with the cells of Sp-2/0 Ag14 mouse myeloma cell line. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3H552 |
| Host | Mouse |
| Concentration | ~0.5mg/ml |
| Form | Supplied as a liquid with 0.1% BSA., 0.09% sodium azide, before addition of glycerol to 40% |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Synthetic peptide corresponding to amino acids 301-318 of rat aquaporin 4. |
| Specificity | Recognizes an epitope within the cytoplasmic domain of the water-specific channel of human aquaporin 4 (AQP4). Does not react with mouse or rat. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
