| Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporins". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. AQP4 (MIWC, mercury-insensitive water channel) is expressed in brain and in other tissues as well. Aquaporin 4 is located in various tissues including the gastro-intestinal tract, kidney and brain. |
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| Catalog # | A3000-14B |
| Applications | Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | ELISA: 1:1000-1:2000 |
| Western Blot: 1:1000-1:10,000; Predicted MW: 38.8kD |
| Immunoprecipitation: 1:1000 -1:10,000 |
| Immunohistochemistry (paraffin): 1:100; Perform heat mediated antigen retrieval with sodium citrate buffer pH 6.0. |
| Immunohistochemistry (frozen): 1:100; Use snap-frozen, acetone-fixed tissue only. |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | Kidney tissue |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 8J301 |
| Host | Mouse |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 1% BSA., 0.09% sodium azide. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Synthetic peptide corresponding to aa301-318 of rat Aquaporin 4. Cellular Localization: Plasma membrane. |
| Specificity | Recognizes an epitope within the cytoplasmic domain of water-specific channel Aquaporin 4. Species Crossreactivity: human. Does not crossreact with rat or mouse. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
