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You are here:Home » Antibodies » Abs to Aquaporin » Anti -Aquaporin 4 (AQP4, WCH4, MIWC, Mercury-insensitive Water Channel)

Anti -Aquaporin 4 (AQP4, WCH4, MIWC, Mercury-insensitive Water Channel)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH
Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporins". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. AQP4 (MIWC, mercury-insensitive water channel) is expressed in brain and in other tissues as well. Aquaporin 4 is located in various tissues including the gastro-intestinal tract, kidney and brain.
Catalog #A3000-14B
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:1000-1:2000
Western Blot: 1:1000-1:10,000; Predicted MW: 38.8kD
Immunoprecipitation: 1:1000 -1:10,000
Immunohistochemistry (paraffin): 1:100; Perform heat mediated antigen retrieval with sodium citrate buffer pH 6.0.
Immunohistochemistry (frozen): 1:100; Use snap-frozen, acetone-fixed tissue only.
Optimal dilutions to be determined by the researcher.
Positive ControlKidney tissue
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No8J301
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 1% BSA., 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa301-318 of rat Aquaporin 4. Cellular Localization: Plasma membrane.
SpecificityRecognizes an epitope within the cytoplasmic domain of water-specific channel Aquaporin 4. Species Crossreactivity: human. Does not crossreact with rat or mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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