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You are here:Home » Antibodies » Abs to Aquaporin » Anti -Aquaporin 6 (WCH3, hKID, AQP2-L)

Anti -Aquaporin 6 (WCH3, hKID, AQP2-L)

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Specifications

Clone Host Grade Applications
Polyclonal Chicken Affinity Purified E B
Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. AQP0 or MIP26 (major intrinsic protein 26kD), and Aquaporin-1 (AQP1, purified from red cells) also called CHIP-28 (channel forming integral protein, 28kD; 268aa; gene locus 7p14) has been the foundation of the growing family of aquaporin. The lens specific AQP0 represents up to 80% of total lens membrane protein. Defects in MIP26 are cause of autosomal dominant cataract. The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP. The lens opacity mutation (LOP) is an amino acid substitution that inhibits targeting of MIP to the cell membrane.
Catalog #A3000-25B
ApplicationsSuitable for use in Immunofluorescence, ELISA, Western Blotting, Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using Chemiluminescence technique.
ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure. Control peptide can be used to coat ELISA plates at 1ug/ml.
Immunohistochemistry & Immunofluorescence: we recommend the use of affinity purified antibody at 2-10ug/ml in paraformaldehyde fixed sections of tissues. Neat serum can be used at 1:500 or more.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer..
We recommend the use of 0.5-1% milk in all primary/secondary antibody-enzyme conjugate incubations in order to suppress non-specific bands.
Clone TypePolyclonal
IsotypeIgY
HostChicken
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.4 and 0.1% BSA, 0.1% sodium azide, 40% glycerol.
PurityPurified over the peptide-Sepharose column
ImmunogenA 19 AA synthetic peptide within the carboxy terminal domain of human AQP6 (1). This domain is predicted to be cytoplasmic.
SpecificityThe 19 AA peptide was found unique to AQP6 without significant homology to any other AQPs. AQP6 from other species are yet to be cloned. Antibody crossreactivity in various species is not known.Control peptides, because of their low MW (<3kD), are not suitable for Western. It should be used for ELISA or antibody blocking to confirm antibody specificity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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