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You are here:Home » Antibodies » Abs to Aquaporin » Anti -Aquaporin 8 (AQP8)

Anti -Aquaporin 8 (AQP8)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH IF
Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein “aquaporin”. Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. Aquaporin-0 or MIP26 (major intrinsic protein 26kD), and Aquaporin-1 (purified from red cells) also called CHIP-28 (channel forming integral protein, 28kD; 268 AA; gene locus 7p14) has been the foundation of the growing family of aquaporins. The lens specific Aquaporin-0 represents up to 80% of total lens membrane protein.
Catalog #A3000-35
ApplicationsSuitable for use in Western Blot, ELISA, Immunohistochemistry and Immunofluorescence. Other applications not tested.
Recommended DilutionWestern Blot: 1-10ug/ml
ELISA: 0.5-1ug/ml
Immunohistochemistry and Immunofluorescence: 2-10ug/ml in a paraformaldehyde fixed sections of tissue.
Optimal dilution to be determined by researcher
NOTE We recommend the use of 0.5-1% milk in all primary/secondary antibody-enzyme conjugate incubations in order to suppress non-specific bands
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
Concentration~1mg/ml
FormSupplied as a lyophilized powder from PBS, pH 7.4, 0.1% BSA. Reconstitute with 100ul of PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenA 16 AA synthetic peptide within the C-terminal, cytoplasmic domain of rat Aquaporin-8 (Accession #P56405). Species sequence homology: 93% mouse, 81% human.
SpecificityRecognizes rat Aquaporin 8.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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