| Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein “aquaporin.” A new water channel, AQP8, has been identified in rat pancreas and testis by homology cloning (1). AQP8 (263aa; 28kD) is also found in liver, colon and salivary glands. AQP8 is expressed in all stages of spermatogenesis. Unlike other AQP, AQP8 has unusually long N-terminus and a short C-terminus. AQP families of proteins are predicted to contain six trans- membrane domains. The N and C-termini are predicted to be cytoplasmic. AQP8 has significant homology to various AQPs: g-TIP (plant water channel, 37%); AQP2 and MIP (37%), AQP1, AQP4, and AQP5 (30-34%); AQP3 (26%). AQP8 does not facilitate glycerol transport. APQ8 forms a mercury-sensitive water-specific channel. It may have an important role in spermatogenesis, in fertilization, and in the secretion of pancreatic juice and saliva. |
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| Catalog # | A3000-36 |
| Applications | Suitable for use in ELISA, Western Blot. Other applications have not been tested. |
| Recommended Dilution | ELISA: 0.5-1.0ug/ml |
| Western Blot: 1-10ug/ml (ECL). Use 0.5-1% milk for primary/secondary antibody enzyme conjugate incubations to suppress non-specific bands. |
| Optimal dilutions to be determined by researcher. |
| Control Peptide | A3000-36L Aquaporin 8, Rat |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Clone Type | Polyclonal |
| Isotype | IgY |
| Host | Chicken |
| Source | Rat |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, 0.1% BSA. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | 16aa synthetic peptide within the C-terminal, cytoplasmic domain of rat Aquaporin-8 (1). |
| Specificity | Recognizes rat Aquaporin-8. The peptide is unique to Aquaporin-8 without significant homology to any other Aquaporins. Species Sequence Homology: mouse, 93%; human, 81%. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
