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You are here:Home » Antibodies » Abs to Aquaporin » Anti -Aquaporin 10 (AQP10)

Anti -Aquaporin 10 (AQP10)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. A new member of AQP family, AQP10, has been cloned (1). AQP10 (human 264 aa) is most closely related with AQP3 (53%), AQP9 (52%), and AQP7 (43%). It is abundantly expressed in duodenum and jejunum. AQP10 is not permeable to urea and glycerol.
Catalog #A3000-80
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionNote: We recommend the use of 0.5-1% milk in all primary/secondary antibody-enzyme conjugate incubations in order to suppress non-specific bands.
Western Blot: 1-10ug/ml using ECL.
ELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Control PeptideA3000-70 Aquaporin 10, Human, Control Peptide (AQP10)
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS,0.1% BSA, 0.1% sodium azide, 40% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenA 17 AA synthetic peptide within the cytoplasmic, C-terminal region of human AQP10 (1) coupled to KLH
SpecificityThe 17 aa peptide is unique to AQP10 without significant homology to any other AQPs. AQP10 from other species are yet to be cloned.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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