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Arachis hypogaea (PNA, Peanut Agglutinin) (Agarose)
Arachis hypogaea (PNA, Peanut Agglutinin) (Agarose)
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| Lectins are derived from the extracts of plants, animals, viruses and microorganisms. They are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms. Lectins form precipitates with glycoconjugates which is useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose. | | | Catalog # | A3305-10 | | Arachis hypogaea (PNA, Peanut Agglutinin), 110kD, is composed of four identical subunits of ~27kD each. It is galactose-specific, with strong anti-T-antigen activity. T-antigen is present in many glycoconjugates such as M and N blood groups, gangliosides, and many other soluble and membrane- associated glycoproteins and glycolipids. PNA does not agglutinate normal human erythrocytes. It strongly agglutinates neuraminidase-treated red blood cells. The presence of Ca++ can enhance the binding of PNA to receptors. Asialo GM1 ganglioside is also thought to be a major cell surface receptor for PNA. PNA is useful in normal and tumor tissue determination for transitional mucosa malignancies. | | CAS Number | N/A | | Molecular Weight | 120kD | | Source | Peanut (PNA) | | | Spacer | None | | | Linkage Between Spacer & Bead | Imidoester | | | Bead Size | 50-250um | | | Quality Control | SDS-PAGE | | | Concentration | 4-5mg purified, PNA/ml settled beads. | | | Form | Supplied as a suspension covalently bound to 4% agarose, PBS, pH 7.2, sodium azide. | | | Carbohydrate for Elution | | | 0.1M lactose or 0.2M galactose in buffer. 10-50mM N-acetylgalactosamine may also be used. | | | Application Note | Calcium is required for binding. 0.5mM calcium is the maximum concentration in buffer that will not form a white precipitate. | | | Storage and Stability | | | Store at 4°C only. Do Not Freeze! Stable for 6 months at 4°C. Further dilutions can be made in assay buffer. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | peanut |
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